TBE buffer (Tris Borate EDTA)
Usage:
- To prepare agarose gel for separating or analyzing DNA fragments.
- As electrolyte during electrophoresis of above DNA fragments.
| component | solubility (g/100ml) (30 ⁰C) |
| Tris base | 50 |
| Boric acid | 6.23 |
| EDTA | see here |
Composition of TBE buffer (10 X, 1litre)
| Component | Amount |
| Tris base | 108 g |
| Boric acid | 55 g |
| 0.5 M EDTA | 40 ml |
Looking at the solubility of boric acid, you can see that preparation of 50X or 100X TBE buffer is not possible.
Working concentration of TBE buffer is 0.5X, i.e. you can prepare agarose gel in 0.5X TBE buffer and also run the gel in 0.5X TBE buffer.
